Cichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach

Natural coumarins are reputed to demonstrate several biological effects improve adverse health conditions including; obesity. This study was conducted explore the potential in vitro lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines . Methods: The were cultured viability assessed. Glucose content medium cells measured. Differentiation from pre-adipocytes adipocytes evaluated upon addition cichorioside. Expression mRNA peroxisome proliferator-activated receptor gamma (PPARγ) estimated. Results: Survival around 90% observed at 10μM Cichorioside inhibited uptake by lines. considerably pre-adipocyte differentiation intercellular storage. demonstrated an upregulation expression PPARγ. Moreover, docking studies supported results via deep interaction with amino acids residue PPAR-γ. Taken together, these findings first report evaluation modulate storage